Interspecies variation of Kitasatospora recifensis endophytic from yam bean producing thermostable amylases in alternative media.
|アブストラクト||An endophytic actinomycete isolated from tubers of yam beam (Pachyrhizus erosus L. Urban) was classified as a novel species nominated Kitasatospora recifensis based in phenotypic and genotypic analysis (16S rDNA gene sequence). Monosporic culture using specific ISP2 media revealed three interspecies, which were identified by DNA southern hybridization (Wild strain 13817 W, Aerial Mycelium strain 13817 AM and Vegetative Mycelium strain 13817 VM). The strains were tested for the production of amylolitic enzymes in alternative media. Maximum yields for both enzymes were observed in starch-casein. Higher alpha-amylase was obtained with strain 13817 W in starch-urea, and amyloglucosidase with strain 13817 AM in starch-ammonium that are economic sources and may be important for industrial purposes. Type strain (DAUFPE 13817(T) = KCTC 9972(T )= DSM 44943(T)).|
|ジャーナル名||World journal of microbiology & biotechnology|
|投稿者||Stamford, Tania Lucia Montenegro; Stamford, Thayza Christina Montenegro; Stamford, Newton Pereira; Santos, Carolina Etienne Rosalia Silva; de Lyra, Maria do Carmo Catanho Pereira; Ha-Park, Yong; Bae, Jin-Won; Araujo, Janete Magali|
|組織名||Department of Nutrition, University Federal of Pernambuco-UFPE, Rua Jader de;Andrade, 335 Casa Forte, 52061-060, Recife, Pernambuco, Brazil.;email@example.com.;University Integrated of Patos, Paraiba, Brazil.;Department of Agronomy, University Federal Rural of Pernambuco-UFRPE, Recife,;Brazil.;Korea Research Institute of Bioscience & Biotechnology, Dae Jeon, Korea.;Department of Anitibiotics, University Federal of Pernambuco, Recife, Brazil.|